Benzo [a] pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children

0518520 - ÚEM 2020 RIV US eng J - Článek v odborném periodiku
Choi, H. - Song, W.M. - Wang, M. - Dostál, Miroslav - Pastorková, Anna - Líbalová, Helena - Tulupová, Elena - Rössnerová, Andrea - Rössner ml., Pavel - Šrám, Radim - Zhang, B.
Benzo [a] pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children.
Environment International. Roč. 128, jul. (2019), s. 218-232. ISSN 0160-4120. E-ISSN 1873-6750
Grant CEP: GA MŠMT(CZ) 2B08005
Institucionální podpora: RVO:68378041
Klíčová slova: polycyclic aromatic hydrocarbon * asthma * air pollution
Obor OECD: Public and environmental health
Impakt faktor: 7.577, rok: 2019
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S0160412018317215?via%3Dihub

Background: The extent to which ambient benzo[a]pyrene (B[a]P) contributes to mechanistically distinct de novo asthma remains unknown.
Objectives: To identify molecular signatures and regulatory networks underlying childhood exposure to ambient B[a]P and asthma, using robust and unbiased systems biology approaches.
Methods: Clinically confirmed asthmatic (n = 191) vs. control (n = 194) children (aged, 7-15) were enrolled from a polluted urban center and semi-rural region in Czech Republic. Contemporaneous B[a]P concentration, gene expressions, DNA methylation data were analyzed against asthma diagnosis, as well as a modified prognostic index of asthma, using integrative multiscale co-expression network analysis. Sample-wise cell type compositions were inferred by a machine learning approach (i.e. CIBERSORT) with reference gene expressions of purified 38 distinct hematopoietic cell states from umbilical cord (i.e. stem cell/progenitors) or peripheral blood (i.e. lymphocytes).
Results: The median outdoor B[a]P was increased near the homes of the urban children with 'moderate' or 'severe' prognostic markers of asthma, but not in the urban controls. An elevated B[a]P induced epigenetic suppression of NF-kappa B inflammation, decreased Natural Killer T (NKT) cells and activated anti-inflammatory IL10-secreting CD8 + T effective memory cells. B[a]P was positively correlated with an increased expression of a heme biosynthesis gene, ALAS2, which in turn, appears to promote concurrent increase of neutrophilic meta-myelocyte and mature CD71(low) erythroid cells. Furthermore, erythroid-specific master transcription regulator gene (GATA1), glutathione transferase genes (GSTM1 and GSTM3) and Eosinophil marker (IL5RA) were simultaneously activated in the urban asthma cases.
Conclusions: B[a]P might contribute to concurrent suppression of pro-inflammatory (e.g. NF-kappa B mediated NKT cells), and activation of anti-inflammatory pathways (e.g. IL10-secreting CD8 + T cells) in the urban asthmatic children. In addition, B[a]P appears to elevate heme biosynthesis, which in turn, promotes neutrophilic meta-myelocyte expansion and reduction of CD71(+) erythroids.
Trvalý link: http://hdl.handle.net/11104/0303642