Catabolism of 8-oxo-purines is mainly routed via the guanine to xanthine interconversion pathway in Mycobacterium smegmatis

0518342 - ÚOCHB 2020 RIV GB eng J - Článek v odborném periodiku
Knejzlík, Zdeněk - Herkommerová, Klára - Pichová, Iva
Catabolism of 8-oxo-purines is mainly routed via the guanine to xanthine interconversion pathway in Mycobacterium smegmatis.
Tuberculosis. Roč. 119, Dec (2019), č. článku 101879. ISSN 1472-9792
Grant CEP: GA MŠMT LO1302
Institucionální podpora: RVO:61388963
Klíčová slova: Mycobacterium smegmatis * purine biosynthesis * purine catabolism * hypoxanthine * xanthine * guanine
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 2.576, rok: 2019
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S1472979219302513?via%3Dihub

Metabolism of purine bases remains poorly understood in the pathogenic bacterium Mycobacterium tuberculosis and closely related, nonpathogenic Mycobacterium smegmatis (Msm). To gain insight into the purine metabolism in mycobacteria, we tested uptake of purine bases with a Delta purF Msm mutant with an inactive purine de novo biosynthesis pathway and confirmed that hypoxanthine and guanine, but not xanthine, can serve as nucleotide precursors for recycling in the salvage pathway. Further, we focused on purine catabolism in wild-type (wt) Msm. We found that only xanthine and guanine could serve as a sole nitrogen source for wt Msm. These data confirm that Msm catabolism of purines is directed mainly via oxidative guanine to xanthine interconversion and not through metabolic conversion of hypoxanthine to xanthine. Our data represent the first experimental evidence confirming the use of 8-oxo-purines as a nitrogen source by Msm.
Trvalý link: http://hdl.handle.net/11104/0303929