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Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii
- 1.0518103 - MBÚ 2020 RIV CH eng J - Článek v odborném periodiku
Zachleder, Vilém - Ivanov, Ivan - Vítová, Milada - Bišová, Kateřina
Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii.
Cells. Roč. 8, č. 10 (2019), č. článku 1237. E-ISSN 2073-4409
Grant CEP: GA MŠMT(CZ) LO1416; GA ČR GA15-09231S
Institucionální podpora: RVO:61388971
Klíčová slova: cell cycle arrest * Chlamydomonas reinhardtii * cyclin-dependent kinase
Obor OECD: Cell biology
Impakt faktor: 4.366, rok: 2019
Způsob publikování: Open access
https://www.mdpi.com/2073-4409/8/10/1237
Temperature is one of the key factors affecting growth and division of algal cells. High temperature inhibits the cell cycle in Chlamydomonas reinhardtii. At 39 degrees C, nuclear and cellular divisions in synchronized cultures were blocked completely, while DNA replication was partly affected. In contrast, growth (cell volume, dry matter, total protein, and RNA) remained unaffected, and starch accumulated at very high levels. The cell cycle arrest could be removed by transfer to 30 degrees C, but a full recovery occurred only in cultures cultivated up to 14 h at 39 degrees C. Thereafter, individual cell cycle processes began to be affected in sequence, daughter cell release, cell division, and DNA replication. Cell cycle arrest was accompanied by high mitotic cyclin-dependent kinase activity that decreased after completion of nuclear and cellular division following transfer to 30 degrees C. Cell cycle arrest was, therefore, not caused by a lack of cyclin-dependent kinase activity but rather a blockage in downstream processes.
Trvalý link: http://hdl.handle.net/11104/0303284
Název souboru Staženo Velikost Komentář Verze Přístup 134_Cell cycle arrest by supraoptimal temperature.pdf 2 1.7 MB Vydavatelský postprint vyžádat
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