Microfluidic diamond biosensor using NV centre charge state detection

0518038 - FZÚ 2020 RIV SG eng C - Konferenční příspěvek (zahraniční konf.)
Krečmarová, M. - Vandenryt, T. - Gulka, M. - Burgeois, E. - Fekete, Ladislav - Hubík, Pavel - Thoelen, R. - Mortet, Vincent - Nesládek, M.
Microfluidic diamond biosensor using NV centre charge state detection.
World Congress on Medical Physics and Biomedical Engineering 2018. Singapore: Springer, 2019 - (Lhotská, L.; Súkupová, L.; Lacković, I.; Ibbott, G.), s. 27-31. IFMBE Proceedings, 68/3. ISBN 978-981-10-9022-6. ISSN 1680-0737.
[World Congress on Medical Physics and Biomedical Engineering 2018. Prague (CZ), 03.06.2018-08.06.2018]
Grant CEP: GA AV ČR(CZ) Fellowship J. E. Purkyně
Grant ostatní: AV ČR(CZ) Fellowship J. E. Purkyně
Program: Fellowship J. E. Purkyně
Institucionální podpora: RVO:68378271
Klíčová slova: nitrogen vacancy * diamond * microfluidics
Obor OECD: Condensed matter physics (including formerly solid state physics, supercond.)

We develop DNA sensors based on charge switching in colour centres in diamond. The presented method allows the combination of luminescence sensor and electrochemical sensor working on the principle of electrochemical impedance spectroscopy (EIS). The structure consists of highly boron doped diamond electrode on which an intrinsic diamond is deposited. This intrinsic layer contains NV colour centres. The device is then embedded in PDMS microfluidic flow cell and covered by a transparent ITO electrode. The switching of the NV centre charge state as a response, on diamond surface termination, is crucial tool for the sensitive charged molecules sensing. We demonstrated high sensitivity of the near surface NV centres on surface charge termination. O- terminated surface results in a preferable NV centre charge state of NV0 or NV−, whereas H- terminated surface leads to non-PL NV+ charge state. By this principle any charged molecule can be detected by a customized surface functionalization.
Trvalý link: http://hdl.handle.net/11104/0303285