In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

0517769 - MBÚ 2020 RIV US eng J - Článek v odborném periodiku
Michalcová, K. - Roychoudhury, S. - Halenar, M. - Tvrdá, E. - Kovačíková, E. - Vašíček, J. - Chrenek, P. - Baldovská, S. - Sanislo, L. - Křen, Vladimír - Kolesárová, A.
In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin.
Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes. Roč. 54, č. 9 (2019), s. 752-757. ISSN 0360-1234. E-ISSN 1532-4109
Grant CEP: GA ČR(CZ) GA18-00150S
Institucionální podpora: RVO:61388971
Klíčová slova: Isoquercitrin * ovarian cancer * TGF-beta 1
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 1.697, rok: 2019
Způsob publikování: Omezený přístup
https://www.tandfonline.com/doi/full/10.1080/03601234.2019.1633214

Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 mu g mL(-1). AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-beta 1 and TGF-beta 1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 mu g mL(-1)) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 mu g mL(-1) led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.
Trvalý link: http://hdl.handle.net/11104/0303175