0517567 - FGÚ 2020 RIV CH eng J - Článek v odborném periodiku
Doubková, Martina - Němčáková, Ivana - Jirka, Ivan - Březina, V. - Bačáková, Lucie
Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells.
Materials. Roč. 12, č. 21 (2019), č. článku 3583. ISSN 1996-1944. E-ISSN 1996-1944
Grant CEP: GA ČR(CZ) GA16-02681S; GA ČR(CZ) GA19-02891S
Institucionální podpora: RVO:67985823 ; RVO:61388955
Klíčová slova: silicalite-1 * zeolite * coating * MG-63 cells * osteoblasts * biocompatibility
Obor OECD: Biomaterials (as related to medical implants, devices, sensors); Physical chemistry (UFCH-W)
Impakt faktor: 3.057, rok: 2019 ; AIS: 0.543, rok: 2019
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.3390/ma12213583DOI: https://doi.org/10.3390/ma12213583

Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.
Trvalý link: http://hdl.handle.net/11104/0302896