The role of nitric oxide during embryonic wound healing

0517499 - BTÚ 2020 RIV US eng J - Článek v odborném periodiku
Abaffy, Pavel - Tománková, Silvie - Naraine, Ravindra - Kubista, Mikael - Šindelka, Radek
The role of nitric oxide during embryonic wound healing.
BMC Genomics. Roč. 20, č. 1 (2019), č. článku 815. ISSN 1471-2164. E-ISSN 1471-2164
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR GA17-24441S
Institucionální podpora: RVO:86652036
Klíčová slova: Xenopus laevis * Nitric oxide * Wound healing * Transcriptome
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.594, rok: 2019
Způsob publikování: Open access
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-6147-6

Background The study of the mechanisms controlling wound healing is an attractive area within the field of biology, with it having a potentially significant impact on the health sector given the current medical burden associated with healing in the elderly population. Healing is a complex process and includes many steps that are regulated by coding and noncoding RNAs, proteins and other molecules. Nitric oxide (NO) is one of these small molecule regulators and its function has already been associated with inflammation and angiogenesis during adult healing. Results Our results showed that NO is also an essential component during embryonic scarless healing and acts via a previously unknown mechanism. NO is mainly produced during the early phase of healing and it is crucial for the expression of genes associated with healing. However, we also observed a late phase of healing, which occurs for several hours after wound closure and takes place under the epidermis and includes tissue remodelling that is dependent on NO. We also found that the NO is associated with multiple cellular metabolic pathways, in particularly the glucose metabolism pathway. This is particular noteworthy as the use of NO donors have already been found to be beneficial for the treatment of chronic healing defects (including those associated with diabetes) and it is possible that its mechanism of action follows those observed during embryonic wound healing. Conclusions Our study describes a new role of NO during healing, which may potentially translate to improved therapeutic treatments, especially for individual suffering with problematic healing.
Trvalý link: http://hdl.handle.net/11104/0302837