Circadian profiling reveals distinct regulation of endocannabinoid system in the rat plasma, liver and adrenal glands by light-dark and feeding cycles

0517481 - FGÚ 2020 RIV NL eng J - Článek v odborném periodiku
Sládek, Martin - Houdek, Pavel - Sumová, Alena
Circadian profiling reveals distinct regulation of endocannabinoid system in the rat plasma, liver and adrenal glands by light-dark and feeding cycles.
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1864, č. 12 (2019), č. článku 158533. ISSN 1388-1981. E-ISSN 1879-2618
Grant CEP: GA ČR(CZ) GA17-14704S
Institucionální podpora: RVO:67985823
Klíčová slova: circadian clock * endocannabinoids * rat liver * adrenal gland * restricted feeding * cannabinoid receptors
Obor OECD: Physiology (including cytology)
Impakt faktor: 4.519, rok: 2019
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.bbalip.2019.158533

Circadian clocks coordinate physiological and behavioral rhythms that allow the organism to anticipate and adapt to daily changes in environment. The clock-driven cellular oscillations are highly tissue specific to efficiently fine-tune local signaling, manage energy use and segregate incompatible processes. In most peripheral tissues, food acts as the main cue that entrains the oscillations to external time. Food intake and energy balance are under control of endocannabinoid (EC) signaling. Despite this obvious link between the circadian and EC systems, evidence for their interaction started to emerge only recently. We used targeted lipidomics to analyze circadian variations in EC tone in rat plasma, liver and adrenal tissue. The results provide the evidence that ECs, monoacylglycerols, N-acylethanolamines and their precursors oscillate with a tissue-specific circadian phase in plasma and liver. We then identified a set of rhythmically expressed genes likely responsible for the variations in EC tissue tone. In contrast to the liver, EC levels did not oscillate in the adrenal glands. Instead, we revealed that local EC receptor genes are under circadian regulation. To explore the impact of metabolic signals on expression of these genes, we used daytime-restricted feeding schedule. We subsequently showed that daytime feeding strongly suppressed liver-expressed fatty acid binding protein 5 (Fabp5) and adrenal-expressed non-canonical endocannabinoid receptors Gpr55 and Trpv1, whereas it upregulated liver-expressed Trpv1 and glycerophosphodiester phosphodiesterase 1 (Gde1). Our results reveal tissue-specific mechanisms involved in interaction between endocannabinoid signaling, circadian system and metabolism.
Trvalý link: http://hdl.handle.net/11104/0302786