Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae

0511867 - ÚFCH JH 2020 RIV NL eng J - Článek v odborném periodiku
Marković, Z. M. - Kováčová, M. - Humpolíček, P. - Budimir, M. D. - Vajďák, J. - Kubát, Pavel - Mičušík, M. - Švajdlenková, H. - Danko, M. - Capáková, Z. - Lehocký, M. - Todorović Marković, B. M. - Špitalský, Z.
Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae.
Photodiagnosis and Photodynamic Therapy. Roč. 26, JUN 2019 (2019), s. 342-349. ISSN 1572-1000. E-ISSN 1873-1597
Grant CEP: GA ČR(CZ) GA19-09721S
Grant ostatní: Ministerstvo školství, mládeže a tělovýchovy (MŠMT)(CZ) LO1504
Institucionální podpora: RVO:61388955
Klíčová slova: singlet oxygen generation * methylene-blue * antimicrobial activity * dots * light * nanoparticles * silicone * violet * Hydrophobic carbon quantum dots
Obor OECD: Physical chemistry
Impakt faktor: 2.894, rok: 2019
Způsob publikování: Omezený přístup

Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.
Trvalý link: http://hdl.handle.net/11104/0302113