The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates

0511739 - ÚSMH 2020 RIV CZ eng J - Článek v odborném periodiku
Mitas, P. - Grus, T. - Lambert, L. - Mlček, M. - Chlup, H. - Honsová, E. - Dohnalová, M. - Suchý, Tomáš - Burgetová, A. - Lindner, J. - Špaček, M.
The Influence of Purification of Carp Collagen Used in a Novel Composite Graft with Sandwich Construction of the Wall on Its Biological Properties and Graft Patency Rates.
Physiological Research. Roč. 68, č. 4 (2019), s. 603-610. ISSN 0862-8408. E-ISSN 1802-9973
Institucionální podpora: RVO:67985891
Klíčová slova: Vascular graft * Collagen * Crosslinking * Extrusion * Composite tube * Patency * Sheep
Obor OECD: Medical engineering
Impakt faktor: 1.655, rok: 2019
Způsob publikování: Open access
https://www.biomed.cas.cz/physiolres/pdf/2019/68_603.pdf

We compared graft outcome between two types of a novel composite three-layer carp-collagen-coated vascular graft in low-flow conditions in a sheep model. Collagen in group A underwent more cycles of purification than in group B in order to increase the ratio between collagen and residual fat. The grafts were implanted end-to-side in both carotid arteries in sheep (14 grafts in 7 sheep in group A, 18 grafts in 9 sheep in group B) and artificially stenosed on the right side. The flow in the grafts in group A decreased from 297 +/- 118 ml/min to 158 +/- 159 ml/min (p=0.041) after placement of the artificial stenosis in group A, and from 330 +/- 164ml/min to 97 +/- 29 ml/min (p=0.0052) in group B (p=0.27 between the groups). From the five surviving animals in group A, both grafts occluded in one animal 3 and 14 days after implantation. In group B, from the six surviving animals, only one graft on the left side remained patent (p=0.0017). Histology showed degradation of the intimal layer in the center with endothelization from the periphery in group A and formation of thick fibrous intimal layer in group B. We conclude that the ratio between collagen and lipid content in the novel three-layer graft plays a critical role in its patency and structural changes in vivo.
Trvalý link: http://hdl.handle.net/11104/0301976