Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs

0511690 - BFÚ 2020 RIV DE eng J - Článek v odborném periodiku
Římánková, Ludmila - Ostatná, Veronika - Bartošík, M.
Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs.
Electroanalysis. Roč. 31, č. 10 (2019), s. 1895-1900. ISSN 1040-0397. E-ISSN 1521-4109
Institucionální podpora: RVO:68081707
Klíčová slova: signal amplification strategy * electrochemical determination * stripping voltammetry * cyclic voltammetry * polarography * chitosan * microRNA
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impakt faktor: 2.544, rok: 2019
Způsob publikování: Omezený přístup
https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201900241

We show for the first time that RNA catalyzes hydrogen evolution reaction at mercury-containing electrodes. We previously showed that DNA is electrocatalytically active, and so we compared heights and potentials of the RNA chronopotentimetric stripping (CPS) peaks with analogous signals of DNA of the same sequence and found out they were very similar. RNA peaks showed differences depending on the RNA base composition. Catalytic nature of CPS peak enabled detection of 25 pM microRNA in electrochemical cell, or 500 pM microRNA in a 5 mu L solution drop (corresponding to 2.5 fmole of microRNA). This finding opens the door for simple, label-free and reagent-less analysis of low concentrations of RNA molecules.
Trvalý link: http://hdl.handle.net/11104/0301909