Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

0511086 - BC 2020 RIV NL eng J - Článek v odborném periodiku
Jaime-Perez, Noelia - Kaftan, David - Bína, David - Bokhari, Syed Nadeem Hussain - Shreedhar, Sowmya - Küpper, Hendrik
Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1860, č. 8 (2019), s. 640-650. ISSN 0005-2728. E-ISSN 1879-2650
Grant CEP: GA MŠMT EF15_003/0000336; GA ČR(CZ) GA17-14024S
Institucionální podpora: RVO:60077344 ; RVO:61388971
Klíčová slova: Bacteriochlorophyll * Purple bacteria * Copper toxicity * Light harvesting complex * Photosynthesis
Obor OECD: Biochemistry and molecular biology; Biochemistry and molecular biology (MBU-M)
Impakt faktor: 3.465, rok: 2019
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S0005272819300568?via%3Dihub

Magnesium (Mg2+) is the ubiquitous metal ion present in chlorophyll and bacteriochlorophyll (BChl), involved in photosystems in photosynthetic organisms. In the present study we investigated targets of toxic copper binding to the photosynthetic apparatus of the anoxygenic purple bacterium Rhodospirillum rubrum. This was done by a combination of in vivo measurements of flash photolysis and fast fluorescence kinetics combined with the analysis of metal binding to pigments and pigment-protein complexes isolated from Cu-stressed cells by HPLC-ICPMS (ICP-sfMS). This work concludes that R. rubrum is highly sensitive to Cu2+, with a strong inhibition of the photosynthetic reaction centres (RCs) already at 2 mu M Cu2+. The inhibition of growth and of RC activity was related to the formation of Cu-containing BChl degradation products that occurred much more in the RC than in LH1. These results suggest that the shift of metal centres in BChl from Mg2+ to Cu2+ can occur in vivo in the RCs of R. rubrum under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of the function of these RCs.
Trvalý link: http://hdl.handle.net/11104/0302010