Noninvasive Phenotyping of Plant–Pathogen Interaction: Consecutive In Situ Imaging of Fluorescing Pseudomonas syringae, Plant Phenolic Fluorescence, and Chlorophyll Fluorescence in Arabidopsis Leaves

0510743 - ÚVGZ 2020 RIV CH eng J - Článek v odborném periodiku
Hupp, S. - Rosenkranz, M. - Bonfig, K. - Pandey, C. - Roitsch, Thomas
Noninvasive Phenotyping of Plant–Pathogen Interaction: Consecutive In Situ Imaging of Fluorescing Pseudomonas syringae, Plant Phenolic Fluorescence, and Chlorophyll Fluorescence in Arabidopsis Leaves.
Frontiers in Plant Science. Roč. 10, OCT 19 (2019), č. článku 1239. ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA MŠMT(CZ) LO1415
Výzkumná infrastruktura: CzeCOS II - 90061
Institucionální podpora: RVO:86652079
Klíčová slova: chlorophyll fluorescence imaging * green fluorescence protein (GFP) * imaging PAM * phenolic compounds * plant–pathogen interaction
Obor OECD: Plant sciences, botany
Impakt faktor: 4.402, rok: 2019
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fpls.2019.01239/full

Plant–pathogen interactions have been widely studied, but mostly from the site of the plant secondary defense. Less is known about the effects of pathogen infection on plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in plants in a noninvasive manner. In the present study, virulent and avirulent Pseudomonas syringae strains were transformed with green fluorescent protein (GFP) to follow the spread of bacteria in vivo by imaging Pulse-Amplitude-Modulation (PAM) fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP-transformed bacteria, PAM chlorophyll fluorescence, and phenolic fluorescence from pathogen-infected plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of pathogen spread as well as phenolic and chlorophyll fluorescence in situ, thus providing a novel means to study complex plant–pathogen interactions and relate the responses of primary and secondary metabolism to pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.
Trvalý link: http://hdl.handle.net/11104/0301143