Label-free CARS microscopy through a multimode fiber endoscope

0510088 - ÚPT 2020 RIV US eng J - Článek v odborném periodiku
Traegaardh, Johanna - Pikálek, Tomáš - Šerý, Mojmír - Meyer, T. - Popp, J. - Čižmár, Tomáš
Label-free CARS microscopy through a multimode fiber endoscope.
Optics Express. Roč. 27, č. 21 (2019), s. 30055-30066. ISSN 1094-4087
Grant CEP: GA MŠMT EF15_003/0000476; GA MŠMT ED0017/01/01; GA MŠMT(CZ) LO1212
Institucionální podpora: RVO:68081731
Klíčová slova: imaging in-vivo * light * tissue
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.669, rok: 2019
Způsob publikování: Open access
https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-27-21-30055&id=421966

Multimode fibres have recently been employed as high-resolution ultra-thin endoscopes, capable of imaging biological structures deep inside tissue in vivo. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fibre endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial 125 mu m diameter, 0.29 NA GRIN fibre, and wavefront shaping on an SLM is used to create foci that are scanned behind the fibre facet across the sample. The chemical selectivity is demonstrated by imaging 2 mu m polystyrene and 2.5 mu m PMMA beads with per pixel integration time as low as 1 ms for epi-detection.
Trvalý link: http://hdl.handle.net/11104/0300644