PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION

0509660 - ÚEB 2020 RIV US eng M - Část monografie knihy
Noack, L. C. - Pejchar, Přemysl - Sekereš, Juraj - Jaillais, Y. - Potocký, Martin
Transient gene expression as a tool to monitor and manipulate the levels of acidic phospholipids in plant cells.
PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. TOTOWA: HUMANA PRESS INC., 2019 - (Cvrčková, F.; Žárský, V.), s. 189-199. Methods in Molecular Biology. ISBN 978-1-4939-9469-4
Grant CEP: GA ČR GA17-27477S; GA ČR(CZ) GC18-18290J; GA ČR(CZ) GA19-21758S
Institucionální podpora: RVO:61389030
Klíčová slova: Microscopy * Nicotiana benthamiana * Nicotiana tabacum * Phosphoinositides * Phospholipid-binding domains * Pollen tube * Transient expression
Obor OECD: Cell biology

Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein–phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
Trvalý link: http://hdl.handle.net/11104/0300327