Počet záznamů: 1  

Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems

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    0509613 - ÚOCHB 2020 RIV NL eng J - Článek v odborném periodiku
    Kryštůfek, Robin - Šácha, Pavel
    Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems.
    MethodsX. Roč. 6, Sep 25 (2019), s. 2230-2236. E-ISSN 2215-0161
    Grant CEP: GA MŠMT(CZ) EF16_019/0000729; GA MPO(CZ) FV10490; GA ČR(CZ) GA19-10280S
    Institucionální podpora: RVO:61388963
    Klíčová slova: acoustic liquid transfer * protein crystallography * crystallization screening * laboratory automation
    Obor OECD: Biochemical research methods
    Způsob publikování: Open access
    https://www.sciencedirect.com/science/article/pii/S2215016119302547?via%3Dihub

    Advances in contactless acoustic liquid transfer technologies have unlocked opportunities to substantially increase the throughput of crystallization screens and decrease the consumption of reagents and consumables. Acoustic energy transfer enables crystallization experiments to be set up precisely and rapidly on a nanoliter scale. Nonetheless, adapting acoustic transfer methods to a diverse range of crystallization conditions and their physicochemical idiosyncrasies remains a major bottleneck for true universality of this technique. Even though the reagent limitations still remain an issue, we present a straightforward protocol for setting up crystallization experiments by acoustic transfer using a Labcyte Echo 550 instrument, with a focus on the technical limitations of this method, including reagent compatibilities, spatial resolution and downscaling limits.
    Trvalý link: http://hdl.handle.net/11104/0300302

     
     
Počet záznamů: 1  

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