Počet záznamů: 1
Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems
- 1.0509613 - ÚOCHB 2020 RIV NL eng J - Článek v odborném periodiku
Kryštůfek, Robin - Šácha, Pavel
Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems.
MethodsX. Roč. 6, Sep 25 (2019), s. 2230-2236. E-ISSN 2215-0161
Grant CEP: GA MŠMT(CZ) EF16_019/0000729; GA MPO(CZ) FV10490; GA ČR(CZ) GA19-10280S
Institucionální podpora: RVO:61388963
Klíčová slova: acoustic liquid transfer * protein crystallography * crystallization screening * laboratory automation
Obor OECD: Biochemical research methods
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S2215016119302547?via%3Dihub
Advances in contactless acoustic liquid transfer technologies have unlocked opportunities to substantially increase the throughput of crystallization screens and decrease the consumption of reagents and consumables. Acoustic energy transfer enables crystallization experiments to be set up precisely and rapidly on a nanoliter scale. Nonetheless, adapting acoustic transfer methods to a diverse range of crystallization conditions and their physicochemical idiosyncrasies remains a major bottleneck for true universality of this technique. Even though the reagent limitations still remain an issue, we present a straightforward protocol for setting up crystallization experiments by acoustic transfer using a Labcyte Echo 550 instrument, with a focus on the technical limitations of this method, including reagent compatibilities, spatial resolution and downscaling limits.
Trvalý link: http://hdl.handle.net/11104/0300302
Počet záznamů: 1