Retention and selectivity of sulfobetaine-stationary phase in methanol- and isopropanol-rich mobile phases

0509167 - ÚIACH 2020 PL eng A - Abstrakt
Moravcová, Dana - Planeta, Josef
Retention and selectivity of sulfobetaine-stationary phase in methanol- and isopropanol-rich mobile phases.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 47-47.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
Grant CEP: GA ČR(CZ) GA19-00742S; GA MV VI20172020069
Institucionální podpora: RVO:68081715
Klíčová slova: capillary monolithic columns * tetramethoxysilane * mobile phases
Obor OECD: Analytical chemistry

The sulfobetaine-capillary monolithic columns (0.1mm x 150mm) were prepared by acidic hydrolysis of tetramethoxysilane in the presence of polyethylene glycol and urea and modified to zwitterionic-stationary phase by [2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide. These columns were used to evaluate an effect of the mobile-phase composition on retention and selectivity of sulfobetaine-stationary phase employing acidic, basic and neutral compounds as test analytes.
The effects of the content of organic solvents (acetonitrile, isopropanol and methanol) or their mixtures in mobile phase on separation of selected analytes were evaluated under isocratic elution conditions. An addition up to 10 % (v/v) of methanol or isopropanol to the acetonitrile-rich mobile phase caused only minor changes in retention and selectivity of sulfobetaine-stationary phase. On the other hand, methanol- as well as isopropanol-rich mobile phases significantly influenced the separation of test analytes.
Trvalý link: http://hdl.handle.net/11104/0299931