Optimization of pressurized hot water extraction for isolation of allergen proteins from almonds

0509085 - ÚIACH 2020 PL eng A - Abstrakt
Burdějová, Lenka - Moravcová, Dana - Strouhalová, Dana - Karásek, Pavel - Roth, Michal - Kudláčková, Barbora
Optimization of pressurized hot water extraction for isolation of allergen proteins from almonds.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 12-12.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
Grant CEP: GA ČR(CZ) GA19-00742S
Institucionální podpora: RVO:68081715
Klíčová slova: pressurized hot water extraction (PHWE) * plant proteins * extraction of allergens
Obor OECD: Analytical chemistry

An increasing demand for rapid, inexpensive and efficient allergen extraction method has emerged in recent years. Pressurized hot water extraction (PHWE) has become popular for plant proteins isolation. In the presented contribution, optimization of PHWE parameters for efficient almond protein allergens isolation was carried out, followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Non-defatted/defatted fine almond powder was mixed with inert glass material in ratio 1:10, packed into 11ml extraction vessel and extracted for 15 minutes at pressure of 15 MPa and with temperature range 40 °C–120 °C to determine the optimal extraction temperature. Further, four extraction times (2, 5, 10 and 15 min) at optimized temperature were tested. Finally, the effect of extraction cycle numbers from 1 to 5 was studied at optimized conditions. It has been found that allergen extraction is more efficient for defatted compared with non-defatted samples. A negative effect on allergen isolation was observed with increasing temperature, 40 °C was optimum for both sample types. Extraction of allergens was quite fast when most of the allergens were extracted within 2 min; no significant increase/decrease in allergens content was detected while the extraction time was prolonged. Additional extraction cycles improved the extraction results minimally, two cycles with duration 2 min were sufficient to maximize allergen recovery. We suggest these optimal PHWE conditions: temperature 40 °C, extraction time 2 min, one cycle of extraction at 15 MPa for both types of samples.
Trvalý link: http://hdl.handle.net/11104/0299876