Epitachophoresis – new way for large sample volume concentration and separation

0509065 - ÚIACH 2020 PL eng A - Abstrakt
Foret, František - Datinská, Vladimíra - Voráčová, Ivona - Novotný, Jakub - Gheibi, P. - Berka, J. - Astier, Y.
Epitachophoresis – new way for large sample volume concentration and separation.
CECE 2019. Gdańsk: University of Gdańsk, 2019. s. 5-5.
[CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./. 24.09.2019-26.09.2019, Gdańsk]
Grant CEP: GA MŠMT(CZ) 8F17003
Institucionální podpora: RVO:68081715
Klíčová slova: epitachophoresis * isotachophoresis * electrophoresis
Obor OECD: Analytical chemistry

Here, we report on a new instrumental system for processing of large sample volumes by discontinuous electrophoresis with theoretically unlimited concentration factor. The laboratory constructed device was designed in a circular arrangement where sample zones migrated towards a fraction collection well in the center. This allowed focusing of 15 ml sample volumes in a 110 mm device in less than 1 hour. Position of the migrating zone was monitored by laser-induced fluorescence, thermal imaging or surface conductivity detectors. While a discontinuous electrolyte system common for isotachophoresis was used, the selected geometry did not lead to a typical isotachophoretic migration with respect to the linear velocity of the zones. For example, in the constant current mode, most commonly used in capillary isotachophoresis, the speed of the migrating zones increased during the analysis. Thus, we propose the name “epitachophoresis” for this mode of separation.
Trvalý link: http://hdl.handle.net/11104/0299860