Archangelolide: A sesquiterpene lactone with immunobiological potential from Laserpitium archangelica

0508820 - ÚOCHB 2020 RIV DE eng J - Článek v odborném periodiku
Rimpelová, S. - Jurášek, M. - Peterková, L. - Bejček, J. - Spiwok, V. - Majdl, M. - Jirásko, M. - Buděšínský, Miloš - Harmatha, Juraj - Kmoníčková, Eva - Drašar, P. - Ruml, T.
Archangelolide: A sesquiterpene lactone with immunobiological potential from Laserpitium archangelica.
Beilstein Journal of Organic Chemistry. Roč. 15, Aug 13 (2019), s. 1933-1944. ISSN 1860-5397. E-ISSN 1860-5397
Grant CEP: GA MŠMT(CZ) LO1304
Institucionální podpora: RVO:61388963 ; RVO:68378041
Klíčová slova: anti-inflammatory properties * archangelolide * dansyl fluorescent conjugate * sarco/endoplasmic reticulum calcium ATPase * sesquiterpene lactone * trilobolide analogue
Obor OECD: Organic chemistry; Pharmacology and pharmacy (UEM-P)
Impakt faktor: 2.622, rok: 2019
Způsob publikování: Open access
https://www.beilstein-journals.org/bjoc/articles/15/189

Sesquiterpene lactones are secondary plant metabolites with sundry biological effects. In plants, they are synthesized, among others, for pesticidal and antimicrobial effects. Two such compounds, archangelolide and trilobolide of the guaianolide type, are structurally similar to the well-known and clinically tested lactone thapsigargin. While trilobolide has already been studied by us and others, there are only scarce reports on the biological activity of archangelolide. Here we present the preparation of its fluorescent derivative based on a dansyl moiety using azide-alkyne Huisgen cycloaddition having obtained the two sesquiterpene lactones from the seeds of Laserpitium archangelica Wulfen using supercritical CO2 extraction. We show that dansyl-archangelolide localizes in the endoplasmic reticulum of living cells similarly to trilobolide, localization in mitochondria was also detected. This led us to a more detailed study of the anticancer potential of archangelolide. Interestingly, we found that neither archangelolide nor its dansyl conjugate did exhibit cytotoxic effects in contrast to the structurally closely related counterparts trilobolide and thapsigargin. We explain this observation by a molecular dynamics simulation, in which, in contrast to trilobolide, archangelolide did not bind into the sarco/endoplasmic reticular calcium ATPase cavity utilized by thapsigargin. Last, but not least, archangelolide exhibited anti-inflammatory activity, which makes it promising compound for medicinal purposes.
Trvalý link: http://hdl.handle.net/11104/0299630