Assessing the Relationship Between Mass Window Width and Retention Time Scheduling on Protein Coverage for Data-Independent Acquisition

0508783 - ÚMG 2020 RIV US eng J - Článek v odborném periodiku
Li, W. - Chi, H. - Šalovská, Barbora - Wu, Ch. - Sun, L. - Rosenberger, G. - Liu, Y.
Assessing the Relationship Between Mass Window Width and Retention Time Scheduling on Protein Coverage for Data-Independent Acquisition.
Journal of the American Society for Mass Spectrometry. Roč. 30, č. 8 (2019), s. 1396-1405. ISSN 1044-0305. E-ISSN 1879-1123
Institucionální podpora: RVO:68378050
Klíčová slova: Data-independent acquisition * Isolation windows * Maxquant * pFind * Spectronaut
Obor OECD: Cell biology
Impakt faktor: 3.255, rok: 2019
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/jasms.8b06074

Due to the technical advances of mass spectrometers, particularly increased scanning speed and higher MS/MS resolution, the use of data-independent acquisition mass spectrometry (DIA-MS) became more popular, which enables high reproducibility in both proteomic identification and quantification. The current DIA-MS methods normally cover a wide mass range, with the aim to target and identify as many peptides and proteins as possible and therefore frequently generate MS/MS spectra of high complexity. In this report, we assessed the performance and benefits of using small windows with, e.g., 5-m/z width across the peptide elution time. We further devised a new DIA method named RTwinDIA that schedules the small isolation windows in different retention time blocks, taking advantage of the fact that larger peptides are normally eluting later in reversed phase chromatography. We assessed the direct proteomic identification by using shotgun database searching tools such as MaxQuant and pFind, and also Spectronaut with an external comprehensive spectral library of human proteins. We conclude that algorithms like pFind have potential in directly analyzing DIA data acquired with small windows, and that the instrumental time and DIA cycle time, if prioritized to be spent on small windows rather than on covering a broad mass range by large windows, will improve the direct proteome coverage for new biological samples and increase the quantitative precision. These results further provide perspectives for the future convergence between DDA and DIA on faster MS analyzers.
Trvalý link: http://hdl.handle.net/11104/0306736