High-proline proteins in experimental hazy white wine produced from partially botrytized grapes

0507851 - ÚEB 2020 RIV US eng J - Článek v odborném periodiku
Perutka, Z. - Šufeisl, M. - Strnad, Miroslav - Šebela, M.
High-proline proteins in experimental hazy white wine produced from partially botrytized grapes.
Biotechnology and Applied Biochemistry. Roč. 66, č. 3 (2019), s. 398-411. ISSN 0885-4513. E-ISSN 1470-8744
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: Botrytis cinerea * proline * proteomics * thaumatin * wine haze * yeast
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 1.638, rok: 2019
Způsob publikování: Open access
http://dx.doi.org/10.1002/bab.1736

Undesirable effects of the pathogen Botrytis cinerea include reduced quality and quantity of wine grapes. Winemaking is also complicated by the formation of a protein haze in white wines and oxidative browning of red wines. We analyzed proteins in experimental Moravian white wines characterized by their instability and haze formation in bottles during storage despite prior bentonite treatment. To study the relationship of wine proteins and haze, we carried out proteomics on hazy and clear white wines produced with partly or largely botrytized grapes and standard reference wines. Wine proteins were identified after their extraction, electrophoresis, and tryptic digestion by reversed-phase liquid chromatography of peptides, coupled with tandem mass spectrometry. Plant defense proteins, yeast glycoproteins, and various enzymes from Botrytis, particularly hydrolases, were found. As the content of the known haze-active thaumatin-like proteins and chitinases was visually low on stained gels (missing bands) compared to previous studies with unfined wines, other proteins are discussed in terms of the haze formation. As the main novelty, this work reveals the role of high proline-containing proteins in the propensity of white wines to turbidity following prior Botrytis damage of grapes.
Trvalý link: http://hdl.handle.net/11104/0298875