Počet záznamů: 1  

A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane

  1. 1.
    0507839 - FGÚ 2020 RIV NZ eng J - Článek v odborném periodiku
    Bačáková, Markéta - Pajorová, Julia - Brož, Antonín - Hadraba, Daniel - Lopot, F. - Zavaďáková, A. - Vištejnová, L. - Beňo, M. - Kostič, I. - Jenčová, V. - Bačáková, Lucie
    A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane.
    International Journal of Nanomedicine. Roč. 14, č. 2019 (2019), s. 5033-5050. E-ISSN 1178-2013
    Grant CEP: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775; GA ČR(CZ) GA17-02448S
    Výzkumná infrastruktura: Czech-BioImaging - 90062
    Institucionální podpora: RVO:67985823
    Klíčová slova: full-thickness skin substitutes * collagen hydrogel * fibroblast and keratinocyte co-cultivation * fibrin * nanostructure
    Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
    Impakt faktor: 5.115, rok: 2019
    Způsob publikování: Open access
    https://doi.org/10.2147/IJN.S200782

    Background: Repairs to deep skin wounds continue to be a difficult issue in clinical practice. A promising approach is to fabricate full-thickness skin substitutes with functions closely similar to those of the natural tissue. For many years, a three-dimensional (3D) collagen hydrogel has been considered to provide a physiological 3D environment for co-cultivation of skin fibroblasts and keratinocytes. This collagen hydrogel is frequently used for fabricating tissue-engineered skin analogues with fibroblasts embedded inside the hydrogel and keratinocytes cultivated on its surface. Despite its unique biological properties, the collagen hydrogel has insufficient stiffness, with a tendency to collapse under the traction forces generated by the embedded cells. Methods: The aim of our study was to develop a two-layer skin construct consisting of a collagen hydrogel reinforced by a nanofibrous poly-L-lactide (PLLA) membrane pre-seeded with fibroblasts. The attractiveness of the membrane for dermal fibroblasts was enhanced by coating it with a thin nanofibrous fibrin mesh. Results: The fibrin mesh promoted the adhesion, proliferation and migration of the fibroblasts upwards into the collagen hydrogel. Moreover, the fibroblasts spontaneously migrating into the collagen hydrogel showed a lower tendency to contract and shrink the hydrogel by their traction forces. The surface of the collagen was seeded with human dermal keratinocytes. The keratinocytes were able to form a basal layer of highly mitotically-active cells, and a suprabasal layer. Conclusion: The two-layer skin construct based on collagen hydrogel with spontaneously immigrated fibroblasts and reinforced by a fibrin-coated nanofibrous membrane seems to be promising for the construction of full-thickness skin substitute.
    Trvalý link: http://hdl.handle.net/11104/0298803

     
     
Počet záznamů: 1  

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