D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain

0507478 - ÚMCH 2020 RIV US eng J - Článek v odborném periodiku
Pongrac, I. M. - Dobrivojević Radmilović, M. - Brkić Ahmed, L. - Mlinarić, H. - Regul, J. - Škokić, S. - Babič, Michal - Horák, Daniel - Hoehn, M. - Gajović, S.
D-mannose-coating of maghemite nanoparticles improved labeling of neural stem cells and allowed their visualization by ex vivo MRI after transplantation in the mouse brain.
Cell Transplantation. Roč. 28, č. 5 (2019), s. 553-567. ISSN 0963-6897. E-ISSN 1555-3892
Grant CEP: GA MŠMT(CZ) LQ1604
GRANT EU: European Commission(XE) 316120 - GLOWBRAIN
Institucionální podpora: RVO:61389013
Klíčová slova: neural stem cells * magnetic resonance imaging * brain
Obor OECD: Polymer science
Impakt faktor: 3.341, rok: 2019
Způsob publikování: Open access
https://journals.sagepub.com/doi/pdf/10.1177/0963689719834304

Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem cells after transplantation. The aim of this study was to (1) evaluate labeling efficiency of D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe2O3)) in neural stem cells (NSCs) in comparison to the uncoated nanoparticles, (2) assess nanoparticle utilization as MRI contrast agent to visualize NSCs transplanted into the mouse brain, and (3) test nanoparticle biocompatibility. D-mannose(γ-Fe2O3) labeled the NSCs better than the uncoated nanoparticles. The labeled cells were visualized by ex vivo MRI and their localization subsequently confirmed on histological sections. Although the progenitor properties and differentiation of the NSCs were not affected by labeling, subtle effects on stem cells could be detected depending on dose increase, including changes in cell proliferation, viability, and neurosphere diameter. D-mannose coating of maghemite nanoparticles improved NSC labeling and allowed for NSC tracking by ex vivo MRI in the mouse brain, but further analysis of the eventual side effects might be necessary before translation to the clinic.
Trvalý link: http://hdl.handle.net/11104/0298801