Počet záznamů: 1
Methinium colorimetric sensors for the determination of cholesterol sulfate in an aqueous medium
- 1.0507410 - ÚMG 2020 RIV CH eng J - Článek v odborném periodiku
Kejik, Z. - Havlík, M. - Bříza, T. - Kaplánek, R. - Dolenský, B. - Králová, Jarmila - Martásek, P. - Král, V.
Methinium colorimetric sensors for the determination of cholesterol sulfate in an aqueous medium.
Sensors and Actuators B - Chemical. Roč. 245, June (2017), s. 1032-1038. E-ISSN 0925-4005
Grant CEP: GA MŠMT(CZ) LQ1604
Institucionální podpora: RVO:68378050
Klíčová slova: Cholesterol sulfate * Sulfated lipids * Steroids * Chemical sensors * Methinium salts * Troger's bases
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.667, rok: 2017
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0925400517301909?via%3Dihub
Methinium colorimetric sensors 1 and 2 were studied for the determination of cholesterol sulfate by UV-Vis spectroscopy in 0.001 M NaCl (H2O:MeOH, 7:3), pH = 7.34. Both of the tested sensors displayed high affinity, excellent selectivity and sensitivity for cholesterol sulfate against other steroids (estrone sulfate, pregnenolone sulfate, cholesterol, cholic acid and taurocholic acid). The determined values of conditional binding constants, linear ranges and detection limits demonstrated the high usability of these sensors for the determination of cholesterol sulfate. For example, the detection limits of tested sensors I and 2 for the detection of cholesterol sulfate were 0.16 mu M (0.07 mg/L, R-2 = 0.9966) and 1.3 mu M (0.59 mg/L, R-2 = 0.9872), respectively. Our detection limit strongly indicates the potential usability of these sensors for the determination of biologically important levels of cholesterol sulfate. (C) 2017 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0298403
Počet záznamů: 1