Inducible protein stabilization system in Leishmania mexicana

0507397 - BC 2020 RIV NL eng J - Článek v odborném periodiku
Podešvová, L. - Huang, H. - Yurchenko, Vyacheslav
Inducible protein stabilization system in Leishmania mexicana.
Molecular and Biochemical Parasitology. Roč. 214, JUN 2017 (2017), s. 62-64. ISSN 0166-6851. E-ISSN 1872-9428
Grant CEP: GA ČR(CZ) GA16-18699S
Institucionální podpora: RVO:60077344
Klíčová slova: gene-expression * Leishmania mexicana * ecDHFR * Trimethoprim * Protein stabilization
Obor OECD: Developmental biology
Impakt faktor: 1.744, rok: 2017
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0166685117300464?via%3Dihub

Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD. (C) 2017 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0298390