WHOLE-GENOME EXPRESSION ANALYSIS IN THP-1 MACROPHAGE-LIKE CELLS EXPOSED TO DIVERSE NANOMATERIALS

0507359 - ÚEM 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Brzicová, Táňa - Líbalová, Helena - Vrbová, Kristýna - Sikorová, Jitka - Philimonenko, Vlada - Kléma, J. - Topinka, Jan - Rössner ml., Pavel
WHOLE-GENOME EXPRESSION ANALYSIS IN THP-1 MACROPHAGE-LIKE CELLS EXPOSED TO DIVERSE NANOMATERIALS.
Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. Ostrava: Tanger Ltd., 2018, s. 679-684. ISBN 9788087294819.
[International Conference on Nanomaterials - Research and Application (NANOCON) /9./. Brno (CZ), 18.10.2017-20.10.2017]
Grant CEP: GA MŠMT(CZ) LM2015073; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) LM2015062
Institucionální podpora: RVO:68378041 ; RVO:68378050
Klíčová slova: nanomaterials * toxicity * THP-1 macrophages * gene expression profiling
Obor OECD: Toxicology; Toxicology (UMG-J)

From the perspective of the immune system, nanomaterials (NMs) represent invading agents. Macrophages are immune cells residing in all organs and tissues as the first line of defense. Interactions of macrophages with NMs can determine the fate of NMs as well as their potential toxic effects. In the present study, we compared toxicity of four different types of NMs [NM-100 (TiO2, 110 nm), NM-110 (ZnO, 20 nm), NM-200 (SiO2, 150 nm) and NM-300K (Ag, 20 nm)], towards THP-1 macrophage-like cells. Cells were incubated with non-cytotoxic concentrations (1-25 mu g/ml) of NMs for 24 hours and microarray technology was used to analyze changes in whole-genome expression. Gene expression profiling revealed a substantially different molecular response following exposure to diverse NMs. While NM-100 did not exert any significant effect on gene expression profile, all other NMs triggered a pro-inflammatory response characterized by an activation of the NF-kappa B transcription factor and induced expression of numerous chemokines and cytokines. NM-110 and NM-300K further modulated processes such as DNA damage response, oxidative and replication stress as well as cell cycle progression and proteasome function. We suppose that genotoxicity of ZnO and Ag NMs leading to DNA damage and alternatively to apoptosis in THP-1 macrophages is probably caused by the extensive intracellular dissolution of these NPs, as confirmed by TEM imaging.
Trvalý link: http://hdl.handle.net/11104/0302802