Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats

0507350 - ÚEM 2020 RIV PL eng J - Článek v odborném periodiku
Růžička, Jiří - Machová-Urdzíková, Lucia - Kloudová, Anna - Amin, A.G. - Vallová, Jana - Kubinová, Šárka - Schmidt, M.H. - Jhanwar-Uniyal, M. - Jendelová, Pavla
Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats.
Acta Neurobiologiae Experimentalis. Roč. 78, č. 4 (2018), s. 358-374. ISSN 0065-1400. E-ISSN 1689-0035
Grant CEP: GA MŠMT(CZ) EF15_003/0000419; GA ČR(CZ) GBP304/12/G069; GA MŠMT(CZ) LTAUSA17120; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LQ1604
Institucionální podpora: RVO:68378041
Klíčová slova: spinal cord injury * human mesenchymal stem cells * curcumin * combined therapy
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 1.529, rok: 2018
Způsob publikování: Open access
https://www.exeley.com/acta_neurobiologiae_experimentalis/doi/10.21307/ane-2018-035

Spinal cord injury leads to a robust inflammatory response that Is an unfavorable environment for stem cell implantation. In this study, we evaluated the effect of combined therapy of curcumin and mesenchymal stem cells (MSC) on behavioral recovery and tissue sparing, glial scar formation, axonal sprouting and inflammatory responses in a rat experimental model of spinal cord injury (SCI). Balloon-induced compression lesion was performed at thoracic (Th8-9) spinal level. Out of the four groups studied, two groups received curcumin on the surface of the spinal cord immediately after SCI and then once a week for 3 weeks together with an intraperitoneal daily curcumin injection for 28 days. The other two groups received saline. Seven days after SCI, human MSC were intrathecally implanted in one curcumin and one saline group. Both curcumin and curcumin combined with MSC treatment improved locomotor ability in comparison to the saline treated animals. The combined treatment group showed additional improvement in advanced locomotor performance. The combined therapy facilitated axonal sprouting, and modulated expression of pro-regenerative factors and inflammatory responses, when compared to saline and single treatments. These results demonstrate that preconditioning with curcumin, prior to the MSC implantation could have a synergic effect in the treatment of experimental SCI.
Trvalý link: http://hdl.handle.net/11104/0302804