Electrochemical Analysis of Sybr Green I and its Interaction with DNA

0506701 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Dudová, Zdenka - Pivoňková, Hana - Havran, Luděk - Fojta, Miroslav
Electrochemical Analysis of Sybr Green I and its Interaction with DNA.
XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Srsenová Lenka - Best Servis, 2015 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2015 (2015), s. 35-37. ISBN 978-80-905221-3-8.
[Moderní elektrochemické metody /35./. Jetřichovice (CZ), 18.05.2015-22.05.2015]
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:68081707
Klíčová slova: gels * Sybr Green I * Pyrolytic graphite electrode * Square wave voltammetry
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.
Trvalý link: http://hdl.handle.net/11104/0297896