Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads

0506687 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Plucnara, Medard - Havran, Luděk - Ecsin, E. - Erdem, A. - Fojta, Miroslav
Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads.
XXXVI. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best servis, 2016 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2016 (2016), s. 161-165. ISBN 978-80-905221-4-5.
[Moderní elektrochemické metody /36./. Jetřichovice (CZ), 23.05.2016-27.05.2016]
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:68081707
Klíčová slova: surface-plasmon resonance * copper ions * Label-free detection
Obor OECD: Physical chemistry

Novel approach of SNP detection in model synthetic oligonucleotides is described.This assay requires only intrinsic electrochemical properties of DNA and except of incorporation of biotin affinity tag, no any labeling or any other modification step is needed. Very good resolution for different variants of polymorphism as well as sufficiently low detection limits were achieved.In combination with using pencil graphited electrode this assay could be very simple and cheap tool for SNP typing.
Trvalý link: http://hdl.handle.net/11104/0297885