Amalgam Electrodes Pretreatment and Electrochemical Analysis of DNA

0506671 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Daňhel, Aleš - Fojta, Miroslav
Amalgam Electrodes Pretreatment and Electrochemical Analysis of DNA.
XXXVI. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best servis, 2016 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2016 (2016), s. 32-36. ISBN 978-80-905221-4-5.
[Moderní elektrochemické metody /36./. Jetřichovice (CZ), 23.05.2016-27.05.2016]
Grant CEP: GA ČR(CZ) GA16-01625S
Institucionální podpora: RVO:68081707
Klíčová slova: double-helical polynucleotides * electrically charged surfaces * mercury-electrode * silver amalgam
Obor OECD: Physical chemistry

Mercury meniscus silver solid amalgam electode (m-AgSAE) represents the most suitable and perspective alternative to mercury electrodes which excel in sensitivity to biopolymers and their structural changes. This work briefly summarizes factors influencing electrochemical analysis of DNA at mercury and amalgam electrodes and it is mainly devoted to development of suitable pretreatment procedure, which could securte repeatable and reliable measurements of DNA at m-AgSAes. The results are essential for further utilization of amalgam electrodes in developement of biosensors, since DNA samples are very strongly adsorbed at the electrodes, and none pretreatment procedure was found to be successful in their complete desorption.
Trvalý link: http://hdl.handle.net/11104/0297870