Utilization of Catalytic Hydrogen Evolution in Electrochemical Analysis of Nucleic Acids

0506628 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Fojta, Miroslav - Daňhel, Aleš - Špaček, Jan - Havran, Luděk - Šebest, Peter - Orság, Petr - Pivoňková, Hana - Vosáhlová, J. - Schwarzová-Pecková, K.
Utilization of Catalytic Hydrogen Evolution in Electrochemical Analysis of Nucleic Acids.
XXXVII. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference. Ústí nad Labem: Best servis, 2017 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2017 (2017), s. 42-45. ISBN 978-80-905221-5-2.
[Moderní elektrochemické metody /37./. Jetřichovice (CZ), 15.05.2017-19.05.2017]
Grant CEP: GA ČR GBP206/12/G151; GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: Catalytic hydrogen evolution * Mercury * Amalgam
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Catalysis of hydrogen evolution (CHE) at mercury in the presence of proteins was discoverd shortly after the introduction of polarography. In contrast, unmodified nucleic acids have not been reported to produce distinct signals due to the CHE to date. Chemically modified nucleic acids bearing certain extrinsic groups produce analytically useful signals due to hydrogen evloution catalyzed by the respective modifications. These species include (a) transition metal complexes, and (b) non-metal catalytically active organic moieties. In addition, the CHE has been reported to be invoved in guanine reduction process at the mercury-based electrodes.
Trvalý link: http://hdl.handle.net/11104/0297841