Reduction of the DNA at a Pyrolytic Graphite Electrode:Opening of the Second Half of the Potential Window

0506589 - BFÚ 2020 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Špaček, Jan - Daňhel, Aleš - Hasoň, Stanislav - Hejduková, M. - Fojta, Miroslav
Reduction of the DNA at a Pyrolytic Graphite Electrode:Opening of the Second Half of the Potential Window.
XXXVIII. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best Servis, 2018 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), Č. 2018 (2018), s. 236-239. ISBN 978-80-905221-6-9.
[Moderní elektrochemické metody /38./. Jetřichovice (CZ), 21.05.2018-25.05.2018]
Grant CEP: GA ČR(CZ) GA16-01625S; GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: 5-Methyleylosine * dna * Electrochemistry * Electrochemical oxidation
Obor OECD: Physical chemistry

The pyrolytic graphite electrode (PGE) can be used at highly negative potentials, providing reduction signals known from mercury-based electrodes. Use of the negative part of the potential window more than doubled the number of analytically useful signals provided by unlabelled DNA on PGE. It enables selective analysis of all canonical DNA bases,5-methylcytosine and uracil It also allows for distinction of overlapping signals cathodic polarization can selectively ´switch off´ DNA anodic signals and rice versa. Newly observed signals corresponding to oxidation of reduced bases and reduction of oxidized bases are also described.
Trvalý link: http://hdl.handle.net/11104/0297814