Trk1-mediated potassium uptake contributes to cell-surface properties and virulence of Candida glabrata

0505661 - FGÚ 2020 RIV GB eng J - Článek v odborném periodiku
Llopis-Torregrosa, Vincent - Vaz, C. - Monteoliva, L. - Ryman, K. - Engstrom, Y. - Gacser, A. - Gil, C. - Ljungdahl, P. O. - Sychrová, Hana
Trk1-mediated potassium uptake contributes to cell-surface properties and virulence of Candida glabrata.
Scientific Reports. Roč. 9, May 17 (2019), č. článku 7529. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA16-03398S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
GRANT EU: European Commission(XE) 606786 - ImResFun
Institucionální podpora: RVO:67985823
Klíčová slova: Candida glabrata * potassium uptake * Trk1 * virulence * adhesion * biofilm formation
Obor OECD: Microbiology
Impakt faktor: 3.998, rok: 2019
Způsob publikování: Open access
https://doi.org/10.1038/s41598-019-43912-1

The absence of high-affinity potassium uptake in Candida glabrata, the consequence of the deletion of the TRK1 gene encoding the sole potassium-specific transporter, has a pleiotropic effect. Here, we show that in addition to changes in basic physiological parameters (e.g., membrane potential and intracellular pH) and decreased tolerance to various cell stresses, the loss of high affinity potassium uptake also alters cell-surface properties, such as an increased hydrophobicity and adherence capacity. The loss of an efficient potassium uptake system results in diminished virulence as assessed by two insect host models, Drosophila melanogaster and Galleria mellonella, and experiments with macrophages. Macrophages kill trk1 Delta cells more effectively than wild type cells. Consistently, macrophages accrue less damage when co-cultured with trk1 Delta mutant cells compared to wild-type cells. We further show that low levels of potassium in the environment increase the adherence of C. glabrata cells to polystyrene and the propensity of C. glabrata cells to form biofilms.
Trvalý link: http://hdl.handle.net/11104/0297091