How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions

0505128 - ÚMG 2020 RIV DE eng J - Článek v odborném periodiku
Bruger, A.M. - Dorhoi, A. - Esendagli, G. - Barczyk-Kahlert, K. - van der Bruggen, P. - Lipoldová, Marie - Perečko, T. - Santibanez, J. - Saraiva, M. - Van Ginderachter, J.A. - Brandau, S.
How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions.
Cancer Immunology and Immunotherapy. Roč. 68, č. 4 (2019), s. 631-644. ISSN 0340-7004. E-ISSN 1432-0851
Institucionální podpora: RVO:68378050
Klíčová slova: Myeloid-derived suppressor cells * T cells * Immunosuppression * Arginase * Proliferation * Mye-EUNITER
Obor OECD: Immunology
Impakt faktor: 5.442, rok: 2019
Způsob publikování: Omezený přístup
https://link.springer.com/article/10.1007%2Fs00262-018-2170-8

Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.
Trvalý link: http://hdl.handle.net/11104/0296645