Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia

0504694 - FGÚ 2020 RIV CZ eng J - Článek v odborném periodiku
Naryzhnaya, N. V. - Khaliulin, I. - Lishmanov, Yu. B. - Suleiman, M.-S. - Tsibulnikov, S.Yu. - Kolář, František - Maslov, L. N.
Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia.
Physiological Research. Roč. 68, č. 2 (2019), s. 245-253. ISSN 0862-8408. E-ISSN 1802-9973
Institucionální podpora: RVO:67985823
Klíčová slova: cardiomyocytes * chronic hypoxia * anoxia/reoxygenation * opioid receptors
Obor OECD: Physiology (including cytology)
Impakt faktor: 1.655, rok: 2019
Způsob publikování: Open access
http://www.biomed.cas.cz/physiolres/pdf/2019/68_245.pdf

We studied the role of the delta, mu, and kappa opioid receptor (OR) subtypes in the cardioprotective effect of chronic continuous normobaric hypoxia (CNH) in the model of acuteanoxia/ reoxygenation of isolated cardiomyocytes. Adaptation of rats to CNH was performed by their exposure to atmosphere containing 12 % of O-2 for 21 days. Anoxia/reoxygenation of cardiomyocytes isolated from normoxic control rats caused the death of 51 % of cells and lactate dehydrogenase (LDH) release. Adaptation of rats to CNH resulted in the anoxia/reoxygenation-induced cardiomyocyte death of only 38 %, and reduced the LDH release. Pre-incubation of the cells with either the non-selective OR blocker naloxone (300 nM/l), the delta OR antagonist TIPP(Psi) (30 nM/l), the selective delta(2) OR antagonist naltriben (1 nM/l) or the mu OR antagonist CTAP (100 nM/l) for 25 minutes before anoxia abolished the reduction of cell death and LDH release afforded by CNH. The antagonist of delta(1) OR BNTX (1 nM/l) or the kappa OR antagonist nor-binaltorphimine (3 nM/l) did not influence the cytoprotective effects of CNH. Taken together, the cytoprotective effect of CNH is associated with the activation of the delta(2 )and mu OR localized on cardiomyocytes.
Trvalý link: http://hdl.handle.net/11104/0296274