Počet záznamů: 1
Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females
- 1.0504101 - ÚŽFG 2020 RIV CH eng J - Článek v odborném periodiku
Končická, Markéta - Tětková, Anna - Jansová, Denisa - del Llano, Edgar - Gahurová, Lenka - Kračmarová, J. - Prokešová, Š. - Mašek, T. - Pospíšek, M. - Bruce, A. W. - Kubelka, Michal - Šušor, Andrej
Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females.
International Journal of Molecular Sciences. Roč. 19, č. 9 (2018), č. článku 2841. E-ISSN 1422-0067
Grant CEP: GA MŠMT EF15_003/0000460; GA ČR GA18-19395S; GA ČR GA15-22765S
Institucionální podpora: RVO:67985904
Klíčová slova: aging * oocyte * MPF
Obor OECD: Reproductive biology (medical aspects to be 3)
Impakt faktor: 4.183, rok: 2018
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/19/9/2841
The rate of chromosome segregation errors that emerge during meiosis I in the mammalian female germ line are known to increase with maternal age, however, little is known about the underlying molecular mechanism. The objective of this study was to analyze meiotic progression of mouse oocytes in relation to maternal age. Using the mouse as a model system, we analyzed the timing of nuclear envelope breakdown and the morphology of the nuclear lamina of oocytes obtained from young (2 months old) and aged females (12 months old). Oocytes obtained from older females display a significantly faster progression through meiosis I compared to the ones obtained from younger females. Furthermore, in oocytes from aged females, lamin A/C structures exhibit rapid phosphorylation and dissociation. Additionally, we also found an increased abundance of MPF components and increased translation of factors controlling translational activity in the oocytes of aged females. In conclusion, the elevated MPF activity observed in aged female oocytes affects precocious meiotic processes that can multifactorially contribute to chromosomal errors in meiosis I.
Trvalý link: http://hdl.handle.net/11104/0295799
Počet záznamů: 1