0504099 - FGÚ 2020 RIV DK eng J - Článek v odborném periodiku
Jágr, Michal - Ergang, Peter - Pataridis, Statis - Kolrosová, M. - Bartoš, M. - Mikšík, Ivan
Proteomic analysis of dentin-enamel junction and adjacent protein-containing enamel matrix layer of healthy human molar teeth.
European Journal of Oral Sciences. Roč. 127, č. 2 (2019), s. 112-121. ISSN 0909-8836. E-ISSN 1600-0722
Grant CEP: GA ČR(CZ) GA17-10832S
Institucionální podpora: RVO:67985823
Klíčová slova: dentin * laser capture microdissection * mass spectrometry * proteome
Obor OECD: Analytical chemistry
Impakt faktor: 2.220, rok: 2019 ; AIS: 0.619, rok: 2019
Způsob publikování: Omezený přístup
Web výsledku:
https://doi.org/10.1111/eos.12594DOI: https://doi.org/10.1111/eos.12594

The dentin-enamel junction (DEJ) is the border where two different mineralized structures - enamel and dentin - meet. The protein-rich DEJ, together with the inner enamel region of mature teeth, is known to exhibit higher fracture toughness and crack growth resistance than bulk phase enamel. However, an explanation for this behavior has been hampered by the lack of compositional information for the DEJ and the adjacent enamel organic matrix (EOM). We studied proteomes of the DEJ and EOM of healthy human molars and compared them with dentin and enamel proteomes from the same teeth. These tissues were cut out of tooth sections by laser capture microdissection, proteins were extracted and cleaved by trypsin, then processed by liquid chromatography coupled to tandem mass spectrometry to analyze the proteome profiles of these tissues. This study identified 46 proteins in DEJ and EOM. The proteins identified have a variety of functions, including calcium ion-binding, formation of extracellular matrix, formation of cytoskeleton, cytoskeletal protein binding, cell adhesion, and transport. Collagens were identified as the most dominant proteins. Tissue-specific proteins, such as ameloblastin and amelogenin, were also detected. Our findings reveal new insight into proteomics of DEJ and EOM, highly mineralized tissues that are obviously difficult to analyze.
Trvalý link: http://hdl.handle.net/11104/0295801