Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of´Solid State Biocatalysis´

0503920 - MBÚ 2020 RIV CH eng J - Článek v odborném periodiku
Kapešová, Jana - Petrásková, Lucie - Markošová, K. - Rebroš, M. - Kotík, Michael - Bojarová, Pavla - Křen, Vladimír
Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of´Solid State Biocatalysis´.
International Journal of Molecular Sciences. Roč. 20, č. 5 (2019), č. článku 1112. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA18-00150S
Institucionální podpora: RVO:61388971
Klíčová slova: Aspergillus niger * rutinosidase * solid-state biocatalysis
Obor OECD: Biochemical research methods
Impakt faktor: 4.556, rok: 2019
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/20/5/1112

Quercetin is a flavonoid largely employed as a phytochemical remedy and a food or dietary supplement. We present here a novel biocatalytic methodology for the preparation of quercetin from plant-derived rutin, with both substrate and product being in mostly an undissolved state during biotransformation. This solid-state enzymatic conversion uses a crude enzyme preparation of recombinant rutinosidase from Aspergillus niger yielding quercetin, which precipitates from virtually insoluble rutin. The process is easily scalable and exhibits an extremely high space-time yield. The procedure has been shown to be robust and was successfully tested with rutin concentrations of up to 300 g/L (ca 0.5 M) at various scales. Using this procedure, pure quercetin is easily obtained by mere filtration of the reaction mixture, followed by washing and drying of the filter cake. Neither co-solvents nor toxic chemicals are used, thus the process can be considered environmentally friendly and the product of bio-quality. Moreover, rare disaccharide rutinose is obtained from the filtrate at a preparatory scale as a valuable side product. These results demonstrate for the first time the efficiency of the Solid-State-Catalysis concept, which is applicable virtually for any biotransformation involving substrates and products of low water solubility.
Trvalý link: http://hdl.handle.net/11104/0295681