Počet záznamů: 1
Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance
- 1.0503850 - FGÚ 2020 RIV GB eng J - Článek v odborném periodiku
Zemančíková, Jana - Papoušková, Klára - Peréz-Torrado, R. - Querol, A. - Sychrová, Hana
Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance.
Letters in Applied Microbiology. Roč. 68, č. 1 (2019), s. 81-86. ISSN 0266-8254. E-ISSN 1472-765X
Grant CEP: GA ČR(CZ) GA15-03708S
GRANT EU: European Commission(XE) 264717 - CORNUCOPIA
Institucionální podpora: RVO:67985823
Klíčová slova: glycerol uptake * intracellular pH * osmotolerance * STL1 * yeast
Obor OECD: Microbiology
Impakt faktor: 2.173, rok: 2019
Způsob publikování: Open access
https://doi.org/10.1111/lam.13093
Saccharomyces kudriavzevii is a nonconventional and rather osmosensitive yeast with a high potential of use in fermentation processes. To elucidate the basis of its relative osmosensitivity, the role of the STL1 gene encoding a putative glycerol uptake system was studied. Under higher osmotic pressure, the addition of a low amount of glycerol to the growth medium improved the growth of S. kudriavzevii and the expression of the STL1 gene was highly induced. Deletion of this gene decreased the strain's ability to grow in the presence of higher concentrations of salts and other solutes. Moreover, the mutant had a disturbed homeostasis of intracellular pH. Expression of the SkSTL1 gene in Saccharomyces cerevisiae complemented the osmosensitivity of the S. cerevisiae hog1 Delta stl1 Delta mutant, and the gene's tagging with GFP localized its product to the plasma membrane. Altogether, a deficiency in glycerol uptake did not seem to be the reason for S. kudriavzevii's low osmotolerance, its Stl1 transporter properly contributes to the regulation of intracellular pH and is crucial to its survival of osmotic stress.
Trvalý link: http://hdl.handle.net/11104/0295646
Počet záznamů: 1