Surface Tension and Intrinsic Amyloid Fluorescence of Serum and Cerebrospinal Fluid Samples in Alzheimer´s Disease

0503659 - ÚI 2020 RIV GB eng J - Článek v odborném periodiku
Gažová, Z. - Bednáriková, Z. - Bartoš, A. - Klaschka, Jan - Krištofíková, Z.
Surface Tension and Intrinsic Amyloid Fluorescence of Serum and Cerebrospinal Fluid Samples in Alzheimer´s Disease.
Biomarkers in Medicine. Roč. 13, č. 4 (2019), s. 267-277. ISSN 1752-0363. E-ISSN 1752-0371
Grant ostatní: GA ČR(CZ) GBP304/12/G069; GA MŠk(CZ) LO1611
Program: GB
Institucionální podpora: RVO:67985807
Klíčová slova: Alzheimer´s disease * amyloid fluorescence * biological samples * biomarkers * normal aging * surface tension
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 2.479, rok: 2019
Způsob publikování: Open access

Aim: Surface tension of biological fluids can be influenced by changes in oligomerization or aggregation of surfactant peptides or proteins. Amphiphilic peptides of amyloid-β or other amyloidogenic peptides/proteins display properties of surfactants, oligomerization and aggregation increase also their fluorescence intensity compared with native structures. Results/methodology: We estimated surface tension and native/ThioflavinT-based/intrinsic amyloid fluorescence intensity in serum and cerebrospinal fluid samples for their evalution as diagnostic biomarkers for Alzheimer´s disease (AD). Discussion/conclusion: Our results indicate that values of surface tension are not a suitable biomarker for AD. However, the ratio of ThioflavinT-based fluorescence to intrinsic amyloid fluorescence in cerebrospinal fluid appears to be an acceptable supportive diagnostic biomarker for AD (its sensitivity was 61.1%, and the specificity 70.8% when compared with aged controls).
Trvalý link: http://hdl.handle.net/11104/0295469