Evidence for non-colligative function of small cryoprotectants in a freeze-tolerant insect

0503490 - BC 2020 RIV GB eng J - Článek v odborném periodiku
Toxopeus, J. - Košťál, Vladimír - Sinclair, B. J.
Evidence for non-colligative function of small cryoprotectants in a freeze-tolerant insect.
Proceedings of the Royal Society B-Biological Sciences. Roč. 286, č. 1899 (2019), č. článku 20190050. ISSN 0962-8452. E-ISSN 1471-2954
Institucionální podpora: RVO:60077344
Klíčová slova: freeze tolerance * cold tolerance * acclimation
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 4.638, rok: 2019
Způsob publikování: Open access
https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2019.0050

We used a combination of metabolomics and manipulative experiments in vivo and ex vivo to examine the function of multiple cryoprotectants in the spring fieldcricket Gryllus veletis. Cold-acclimated G. veletis are freeze-tolerant and accumulate myo-inositol, proline and trehalose in their haemolymph and fat body. Our study is the first to experimentally demonstrate the importance of non-colligative cryoprotectant function for insect freeze toleranceboth in vivo and ex vivo, with implications for choosing new molecules for
cryopreservation.
Trvalý link: http://hdl.handle.net/11104/0296833