Speciation analysis of elements accumulated in .i.Cystoderma carcharias./i. from clean and smelter-polluted sites

0503440 - GLÚ 2019 RIV NL eng J - Článek v odborném periodiku
Borovička, Jan - Braeuer, S. - Sácký, J. - Kameník, J. - Goessler, W. - Trubač, J. - Strnad, L. - Rohovec, Jan - Leonhardt, T. - Kotrba, P.
Speciation analysis of elements accumulated in Cystoderma carcharias from clean and smelter-polluted sites.
Science of the Total Environment. Roč. 648, 15 January (2019), s. 1570-1581. ISSN 0048-9697. E-ISSN 1879-1026
Grant CEP: GA ČR(CZ) GA16-15065S
Institucionální podpora: RVO:67985831
Klíčová slova: hyperaccumulation * cadmium * arsenic species * copper * zinc * selenium
Obor OECD: Environmental sciences (social aspects to be 5.7)
Impakt faktor: 6.551, rok: 2019

Collections of Cystoderma carcharias sporocarps were sampled from clean and smelter-polluted sites and analyzed for Ag, As, Cd, Cu, Pb, Se, and Zn contents. Concentrations of all elements were significantly higher in samples from the smelter-polluted area. Except for As and Pb, all elements were effectively accumulated in the sporocarps at both clean and polluted sites. With the highest concentration of 604 mg Cd kg^-1, C. carcharias can be considered as Cd hyperaccumulator. As revealed by HPLC-ICPQQQMS analysis, the As species in sporocarps from clean and polluted areas involved besides the major arsenobetaine a variety of known and unknown arsenicals, the occurrence of dimethylarsinoylacetate and trimethylarsoniopropionate is reported for the first time for gilled fungi (Agaricales). Size-exclusion chromatography of C. carcharias extracts supported by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and RP-HPLC data indicated that detoxification of intracellular Cd and Cu may largely rely on metallothioneins (MT) or MT-like peptides, not phytochelatins.
Trvalý link: http://hdl.handle.net/11104/0295262