Počet záznamů: 1  

A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes

  1. 1.
    0503431 - BC 2019 RIV GB eng J - Článek v odborném periodiku
    Pyrihová, E. - Motyčková, A. - Voleman, L. - Wandyszewska, N. - Fišer, R. - Seydlová, G. - Roger, A. - Kolísko, Martin - Doležal, P.
    A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes.
    Genome Biology and Evolution. Roč. 10, č. 10 (2018), s. 2813-2822. ISSN 1759-6653. E-ISSN 1759-6653
    Grant CEP: GA ČR(CZ) GA18-28103S; GA MŠMT(CZ) EF16_019/0000759
    Institucionální podpora: RVO:60077344
    Klíčová slova: mitochondrial inner membrane * evolution * hydrogenosomes * extraction * prediction * organelles * insertion * topology * sequence * platform * mitochondrial evolution * protein transport * TIM translocase * Tim17 * mitosomes * Giardia * anaerobic protists
    Obor OECD: Microbiology
    Impakt faktor: 3.726, rok: 2018

    Mitochondria have evolved diverse forms across eukaryotic diversity in adaptation to anoxia. Mitosomes are the simplest and the least well-studied type of anaerobic mitochondria. Transport of proteins via TIM complexes, composed of three proteins of the Tim17 protein family (Tim17/22/23), is one of the key unifying aspects of mitochondria and mitochondria-derived organelles. However, multiple experimental and bioinformatic attempts have so far failed to identify the nature of TIM in mitosomes of the anaerobic metamonad protist, Giardia intestinalis, one of the few experimental models for mitosome biology. Here, we present the identification of a single G. intestinalis Tim17 protein (GiTim17), made possible only by the implementation of a metamonad-specific hidden Markov model. While very divergent in primary sequence and in predicted membrane topology, experimental data suggest that GiTim17 is an inner membrane mitosomal protein, forming a disulphide-linked dimer. We suggest that the peculiar GiTim17 sequence reflects adaptation to the unusual, detergent resistant, inner mitosomal membrane. Specific pull-down experiments indicate interaction of GiTim17 with mitosomal Tim44, the tethering component of the import motor complex. Analysis of TIM complexes across eukaryote diversity suggests that a single Tim translocase is a convergent adaptation of mitosomes in anaerobic protists, with Tim22 and Tim17 (but not Tim23), providing the protein backbone.
    Trvalý link: http://hdl.handle.net/11104/0295242

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.