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Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes

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    0502203 - ÚMG 2019 RIV US eng J - Článek v odborném periodiku
    Jobe, N.P. - Živicová, V. - Mifkova, A. - Rosel, D. - Dvořánková, B. - Kodet, O. - Strnad, Hynek - Kolář, Michal - Sedo, A. - Smetana, K. - Strnadova, K. - Brabek, J. - Lacina, L.
    Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes.
    Histochemistry and Cell Biology. Roč. 149, č. 5 (2018), s. 503-516. ISSN 0948-6143. E-ISSN 1432-119X
    Grant CEP: GA MZd(CZ) NV16-29032A; GA MŠk(CZ) LQ1604; GA MŠk(CZ) ED1.1.00/02.0109
    Institucionální podpora: RVO:68378050
    Klíčová slova: Cancer-associated fibroblasts * Keratinocytes * Cancer microenvironment * Cytokine * Chemokine * Melanoma
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 2.640, rok: 2018

    Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm(2) fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.
    Trvalý link: http://hdl.handle.net/11104/0294147

     
     
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