Exploring the critical factors limiting polyaniline biocompatibility

0501735 - ÚMCH 2020 RIV CH eng J - Článek v odborném periodiku
Kašpárková, V. - Humpolíček, P. - Stejskal, Jaroslav - Capáková, Z. - Bober, Patrycja - Skopalová, K. - Lehocký, M.
Exploring the critical factors limiting polyaniline biocompatibility.
Polymers. Roč. 11, č. 2 (2019), s. 1-12, č. článku 362. E-ISSN 2073-4360
Grant CEP: GA ČR(CZ) GA17-05095S
Institucionální podpora: RVO:61389013
Klíčová slova: polyaniline * monomer * oligomer
Obor OECD: Polymer science
Impakt faktor: 3.426, rok: 2019
Způsob publikování: Open access
https://www.mdpi.com/2073-4360/11/2/362/pdf

Today, the application of polyaniline in biomedicine is widely discussed. However, information about impurities released from polyaniline and about the cytotoxicity of its precursors aniline, aniline hydrochloride, and ammonium persulfate are scarce. Therefore, cytotoxicity thresholds for the individual precursors and their combinations were determined (MTT assay) and the type of cell death caused by exposition to the precursors was identified using flow-cytometry. Tests on fibroblasts revealed higher cytotoxicity of ammonium persulfate than aniline hydrochloride. Thanks to the synergic effect, both monomers in combination enhanced their cytotoxicities compared with individual substances. Thereafter, cytotoxicity of polyaniline doped with different acids (sulfuric, nitric, phosphoric, hydrochloric, and methanesulfonic) was determined and correlated with impurities present in respective sample (HPLC). The lowest cytotoxicity showed polyaniline doped with phosphoric acid (followed by sulfuric, methanesulfonic, and nitric acid). Cytotoxicity of polyaniline was mainly attributed to the presence of residual ammonium persulfate and low-molecular-weight polar substances. This is crucial information with respect to the purification of polyaniline and production of its cytocompatible form.
Trvalý link: http://hdl.handle.net/11104/0294720