Optical Mapping Can Reveal Two-Dimensional Morphology of Atrioventricular Insulation in the Foetal Avian Heart

0501676 - FGÚ 2019 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Olejníčková, Veronika - Janoušek, O. - Ronzhina, M. - Hejč, J. - Zábrodská, E. - Kvasilová, A. - Sedmera, David
Optical Mapping Can Reveal Two-Dimensional Morphology of Atrioventricular Insulation in the Foetal Avian Heart.
Computing in Cardiology 2018. Vol. 45. New York: IEEE, 2018. E-ISSN 2325-887X.
[Computing in Cardiology /45./. Maastricht (NL), 23.09.2018-26.09.2018]
Institucionální podpora: RVO:67985823
Klíčová slova: ventricular pre-excitation * atrio-ventricular re-entry * embryonic heart * optical mapping
Obor OECD: Cardiac and Cardiovascular systems
http://www.cinc.org/cinc-papers-on-line/

Syndromes of ventricular pre-excitation or atrio-ventricular (AV) re-entry are believed to results from inappropriate formation of the AV junction (AVJ) during prenatal development. However, technical limitations pose challenge to study foetal heart electrophysiology and are hampering understanding formation of accessory pathways (APs) bypassing AVJ. Here, we present a novel approach of retrograde perfusion for optical mapping of chicken foetal heart. Together with embryonic heart mapping, we were able to investigate AVJ electrical remodelling during the entire prenatal period. AV electrical insulation was observed concomitant to functional deployment of the conduction system at the time of ventricular septation. However, persistence of ectopic AV electrical continuity was still detected in post-septation foetal hearts. APs registered in 40% of prenatal day 13 (localised in the central anterior or left posterior side) were progressively resolved and none were detected on prenatal day 18. The optical mapping approach thus proved its ability to track AVJ electrical remodelling during whole prenatal chicken development and spatiotemporally localise electrically active APs.
Trvalý link: http://hdl.handle.net/11104/0293670