Phosphatidylinositol 4-kinase III beta (PI4KB) forms highly flexible heterocomplexes that include ACBD3, 14-3-3, and Rab11 proteins

0501576 - ÚOCHB 2020 RIV GB eng J - Článek v odborném periodiku
Chalupská, Dominika - Rozycki, B. - Humpolíčková, Jana - Faltová, L. - Klíma, Martin - Bouřa, Evžen
Phosphatidylinositol 4-kinase III beta (PI4KB) forms highly flexible heterocomplexes that include ACBD3, 14-3-3, and Rab11 proteins.
Scientific Reports. Roč. 9, Jan 24 (2019), č. článku 567. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA17-05200S
Institucionální podpora: RVO:61388963
Klíčová slova: crystal structure * structural basis * ESCRT-I
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.998, rok: 2019
Způsob publikování: Open access
https://www.nature.com/articles/s41598-018-37158-6

Phosphatidylinositol 4-kinase III beta (PI4KB) is a key enzyme of the Golgi system because it produces its lipid hallmark the phosphatidylinositol 4-phosphate (PI4P). It is recruited to Golgi by the Golgi resident ACBD3 protein, regulated by 14-3-3 proteins and it also serves as an adaptor because it recruits the small GTPase Rab11. Here, we analyzed the protein complexes formed by PI4KB in vitro using small angle x-ray scattering (SAXS) and we discovered that these protein complexes are highly flexible. The 14-3-3:PI4KB:Rab11 protein complex has 2:1:1 stoichiometry and its different conformations are rather compact, however, the ACBD3:PI4KB protein complex has both, very compact and very extended conformations. Furthermore, in vitro reconstitution revealed that the membrane is necessary for the formation of ACBD3:PI4KB:Rab11 protein complex at physiological (nanomolar) concentrations.
Trvalý link: http://hdl.handle.net/11104/0296378