Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells

0501540 - FZÚ 2019 RIV US eng J - Článek v odborném periodiku
Perez-Guaita, D. - Kochan, K. - Batty, M. - Doerig, C. - Garcia-Bustos, J. - Espinoza Herrera, Shirly J. - McNaughton, D. - Heraud, P. - Wood, Bayden R.
Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells.
Analytical Chemistry. Roč. 90, č. 5 (2018), s. 3140-3148. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA MŠMT EF15_003/0000447; GA MŠMT LQ1606
Grant ostatní: OP VVV - ELIBIO(XE) CZ.02.1.01/0.0/0.0/15_003/0000447
Institucionální podpora: RVO:68378271
Klíčová slova: IR spectroscopy * structural lipids * AFM-IR * hemozoin * proteins * quantification * localization * conformation * resolution * parasite
Obor OECD: Fluids and plasma physics (including surface physics)
Impakt faktor: 6.350, rok: 2018

Atomic force microscopy-infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nanoscale. AFM-IR maps are acquired using a single wavenumber value: they show either the absorbance plotted against a single wavenumber value or a ratio of two absorbance values. Here, we implement multivariate image analysis to generate multivariate AFM-IR maps and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development. This was achieved by converting the discrete spectral points into a multispectral line spectrum prior to multivariate image reconstruction. The approach was used to generate compositional maps of subcellular structures in the parasites, including the food vacuole, lipid inclusions, and the nucleus, on the basis of the intensity of hemozoin, hemoglobin, lipid, and DNA IR marker bands, respectively.
Trvalý link: http://hdl.handle.net/11104/0293555